Srinath Mote
Hi Bindu,
please have a look at the file in the attachment. It can provide you with suggestions how to prepare a standard curve for qPCR experiments.
Regards, Christian
First you need to purify DNA using standard kit
Then do determination using spectrometer
Make serial dilution and determinate the fluorescente intensity versus dna concentration
Than you get y=mx+b equation
You can use PEG precipitation.
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