Animal Cell Culture Stablisation
Hi,
can you perhaps give some details? Media and serum additives as well as flask characteristics all influence cell attachment and growth.
What is the most favorable passage no of cell lines used for experimentation to get better results and effective estimation?
10 November 2013 2,542 2 View
I have successfully established MIN6 cell Culture and now planing to do further experiments with cell viability assay and cell cytotoxicity assay. For this I am asking for guidance on protocols....
08 September 2013 6,607 11 View
I just want to know whether it is necessary to add antibiotic to cell culture media?
07 August 2013 1,403 17 View
We are going to study secondary messenger antagonist effects in respect to calcium mobilisation in a pancreatic beta cell specific cell line i.e. Pancreatic beta MIN6 cell line I also want to...
31 December 2012 331 8 View
I have encountered problem to observe the actual growth of the cell
08 September 2012 3,497 4 View
Or in what condition cells are unaffected during transportation and what type of measure should we taken?
01 February 2012 7,746 17 View
Cell culture related problems
11 December 2011 4,055 7 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View