Will cell lines loose their physicochemical properties with subsequent passages?

What is the most favorable passage no of cell lines used for experimentation to get better results and effective estimation?

10 November 2013 2,460 2 View

How can we measure the cell density and cell seeding per well?

I have successfully established MIN6 cell Culture and now planing to do further experiments with cell viability assay and cell cytotoxicity assay. For this I am asking for guidance on protocols....

08 September 2013 6,507 11 View

Ideal quantity and % of antibiotic used in media for animal cell culture?

I just want to know whether it is necessary to add antibiotic to cell culture media?

07 August 2013 1,299 17 View

Is there any complete cell culture media specific for the MIN6 cell culture?

We are going to study secondary messenger antagonist effects in respect to calcium mobilisation in a pancreatic beta cell specific cell line i.e. Pancreatic beta MIN6 cell line I also want to...

31 December 2012 244 8 View

Can the growth of different cell line be observed/monitored under inverted microscope? I am unable to judge their growth, can anyone recommend the most suitable microscope type to proceed with my experiment on cell lines?

I have encountered problem to observe the actual growth of the cell

08 September 2012 3,402 4 View

I want to bring my cell line to other city but don't know that if it is possible. If yes, then how?

Or in what condition cells are unaffected during transportation and what type of measure should we taken?

01 February 2012 7,660 17 View

Can be recover the cell lines again ones they get infected. If yes then how and if no than why?

Cell culture related problems

11 December 2011 3,971 7 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

No title

02 March 2021 3,060 3 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View