Shiv kumar Mishra

8 Questions 28 Answers 0 Followers

Questions related from Shiv kumar Mishra

Will cell lines loose their physicochemical properties with subsequent passages?

What is the most favorable passage no of cell lines used for experimentation to get better results and effective estimation?

11 November 2013 2,505 2 View

How can we measure the cell density and cell seeding per well?

I have successfully established MIN6 cell Culture and now planing to do further experiments with cell viability assay and cell cytotoxicity assay. For this I am asking for guidance on protocols....

09 September 2013 6,559 11 View

Ideal quantity and % of antibiotic used in media for animal cell culture?

I just want to know whether it is necessary to add antibiotic to cell culture media?

08 August 2013 1,349 17 View

How can we stablize the primary cell culture of isolated islet cells from rats and further succesfully proceed for secondary and subsequent subculture?

Animal Cell Culture Stablisation

05 May 2013 5,540 1 View

Is there any complete cell culture media specific for the MIN6 cell culture?

We are going to study secondary messenger antagonist effects in respect to calcium mobilisation in a pancreatic beta cell specific cell line i.e. Pancreatic beta MIN6 cell line I also want to...

01 January 2013 289 8 View

Can the growth of different cell line be observed/monitored under inverted microscope? I am unable to judge their growth, can anyone recommend the most suitable microscope type to proceed with my experiment on cell lines?

I have encountered problem to observe the actual growth of the cell

09 September 2012 3,453 4 View

I want to bring my cell line to other city but don't know that if it is possible. If yes, then how?

Or in what condition cells are unaffected during transportation and what type of measure should we taken?

02 February 2012 7,702 17 View

Can be recover the cell lines again ones they get infected. If yes then how and if no than why?

Cell culture related problems

12 December 2011 4,015 7 View