I'd have to say that depends on the source of the infection. Mycoplasma infections are notoriously difficult to detect and nearly impossible to eliminate. If it's a fairly typical contamination event, I found that they could usually be treated with either a different antibiotic (recommended; so if you typically use penicillin/streptomycin in your culture media, try gentamicin) or a higher concentration of FRESH stock (I've used up to 2X). I started by washing my cells in sterile PBS after removing the contaminated media and then incubating them for 30 min in PBS with 2X antibiotics (if it's a fungal/yeast infection, I used 2X amphotericin-B). Then I placed the cells back in normal culture medium with fresh antibiotics. If it doesn't work the first time, it's probably not going to. This is risky and may change some characteristics of your cell line such as growth rate, cell shape, viral susceptibility, or other biochemical characteristics. It may not work for all infections/contaminations. It may kill your cells. It's osmotically stressful--so don't try this for cells you need to use immediately; they will need some time to recover (for example, after you passage them the next time).
In general, it is best to discard contaminated cells, clean EVERYTHING thoroughly (bleach where you can, 70% ethanol where you can't like incubator)., dispose of any old/open media and start fresh.
In general, I'd only recommend trying to salvage a cell line if you have very low stocks or a rare cell line, and then you should probably contact the source (whoever provided the cells) for recommendations.
I'd have to say that depends on the source of the infection. Mycoplasma infections are notoriously difficult to detect and nearly impossible to eliminate. If it's a fairly typical contamination event, I found that they could usually be treated with either a different antibiotic (recommended; so if you typically use penicillin/streptomycin in your culture media, try gentamicin) or a higher concentration of FRESH stock (I've used up to 2X). I started by washing my cells in sterile PBS after removing the contaminated media and then incubating them for 30 min in PBS with 2X antibiotics (if it's a fungal/yeast infection, I used 2X amphotericin-B). Then I placed the cells back in normal culture medium with fresh antibiotics. If it doesn't work the first time, it's probably not going to. This is risky and may change some characteristics of your cell line such as growth rate, cell shape, viral susceptibility, or other biochemical characteristics. It may not work for all infections/contaminations. It may kill your cells. It's osmotically stressful--so don't try this for cells you need to use immediately; they will need some time to recover (for example, after you passage them the next time).
In general, it is best to discard contaminated cells, clean EVERYTHING thoroughly (bleach where you can, 70% ethanol where you can't like incubator)., dispose of any old/open media and start fresh.
In general, I'd only recommend trying to salvage a cell line if you have very low stocks or a rare cell line, and then you should probably contact the source (whoever provided the cells) for recommendations.
I agree with Christina Steel. It is better to get rid of the cell lines once, they are infected with Mycoplasma.
I suggest always use disposable pippets and media containers during the cell culture. Never wash and use them again. that will increase the possibility of Endotoxin infection to the cell lines,
I would say it depends on degree of contamination, if it doesn't affect the cell function properties in which you are interested in then, you can let go, unless if you sell this as a commercia product. In R&D level it is likely to get contaminated, but you no need to bother about it unless it hinders the results of your assay.
I think treatment of cells with different antibiotics and antimycotics for a short time will solve the problem
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