How can we develop robust & scalable strategies for precise gene editing in pluripotent stem cells, allowing us to correct disease-causing mutation ?

13 June 2023 0 7K Report

Even though we have advanced so much into using Genome with Technology to cure diseases, still newborn babies are suffering of abnormal genetic mutations. How can we scale these methods to make them available for the masses ?

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Science method

More Manas Tripurana's questions See All

XRD peak deviation?

In XRD analysis, when we move to a higher angle, like above 50 degrees, if I have two reference lines of different phases, one matches the peak and the other is towards the right of the peak with...

31 March 2024 1,812 2 View

Will the temperature of PCR lid affect the overnight ligation at 16 degree celsius?

I had set up overnight ligation reaction at 16 degree celsius in a PCR machine but the lid temperature was high around 95 degrees. Will it affect the efficeincy of ligation if done in PCR in this way?

22 January 2024 2,587 2 View

Is it possible for yttrium or yttrium oxide to react at 760 degree Celsius during casting of magnesium, if its added to reinforce the magnesium?

Casting was done under SF6-CO2 and argon gases environment.

02 December 2023 4,920 2 View

Is there any non kit based method to isolate PCR amplified product?

Is there any manual method to precipitate PCR amplified product?

01 December 2023 5,539 4 View

For doing blunt end cloning shall I dephosphorylate the linearized vector before blunting?

To ligate blunt insert into blunt vector does the vector need to be dephosphorylated at the time of ligation? I have digested my vector with restriction enzyme that create sticky ends that...

15 November 2023 448 0 View

Will the gene under EF1a promoter (from mammalian expression vector) be expressed in any bacterial host?

I have inserted a gene in downstream to Ef1a promoter of my mammalian expression vector. Can I check the protein expression using any bacterial expression host? Or, will it only express in any...

06 November 2023 3,410 4 View

What are the odds that, the amplicon generated using primers carrying overhangs will have blunt end and does not carry any overhangs ?

The DNA polymerase fills in the gap due to overhangs (using a pair of primers carrying overhangs) during the 2nd round of replication in PCR. Does those gaps actually get filled or they have...

02 November 2023 6,400 1 View

How can I ligate an insert digested with same restriction enzyme from both end to a vector digested with same enzyme?

I have insert having flanking regions with XbaI site and and a vector digested with XbaI. How can I prevent self ligation in insert as well as vector before ligation?

31 October 2023 8,865 1 View

What are the potential risks and ethical considerations associated with intentional release of genetically modified organisms into the environment ?

The question asks about the potential factors of how GMOs affect the food chain and the natural balance of evolution and how can we mitigate these risks?

12 June 2023 5,636 5 View

How to clean the corrosion products from Co-Cr base alloy?

13 May 2022 6,078 8 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

What is the role of oxidative stress in toxin-induced carcinogenesis?

What is the role of oxidative stress in toxin-induced carcinogenesis? I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from...

02 August 2024 3,174 1 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View