How antibodies are designed plz explain in step??

from where and how we select sequence of antibody for designing of antibody??i don't understand .kindly tell me.

19 September 2020 2,649 1 View

Spatial root distribution in intercropping?

hello ever one can some share their ideas or research articles on the below mention topics in an intercropping system? 1. spatial root distribution in inetrcropping? 2. relationship between...

29 March 2018 3,594 2 View

How and why oceanic plate sub-ducts beneath continental plate?

Geologists

10 May 2017 799 2 View

Is there any technology for getting fundus image for choroid layer of an eye?

Already, we have fundus image for retinal layer. similarly, is there technology available for getting fundus image for choroidal layer of an eye. segmented and stacked OCT B-scans can give 3D...

21 February 2016 8,612 2 View

Any suggestion on modelling orthotropic plate in APDL urgently?

13 December 2015 4,275 1 View

Are there any studies or algos that helps to analyse soundness of English writing grammatically?

I am trying to measure the soundness of research papers. In order to do that, I first need to know that are they grammatically sound. Do some studies/algorithms exist in literature for this...

21 September 2015 2,708 5 View

How does one find threshold for the data that follows chi-square distribution with known degree of freedom?

13 July 2014 9,207 1 View

Hii Dear All, I am working on nanopartilces and their Biological applications. I want to check the bacterial DNA damage of nano particles?

01 January 1970 2,897 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View