23 October 2018 3 9K Report

Is RNA electrophoresis a best solution? If yes can anyone guide me the optimized protocol to perform RNA electrophoresis?

More Sitara Nasar's questions See All
Does binding of a protein with cellular receptor at different binding sites activates the corresponding pathway?

I have two different proteins that are predicted to have an affinity with a cellular receptor (i.e., TLR4), however, their binding sites are different (one protein binds at the head of the...

12 January 2023 653 5 View

What does negative to positive transition of magnetoresistance indicate?

I have a semiconducting material that is diamagnetic in nature. On doping with magnetic ions, it shows paramagnetic behaviour. Further on substituting a non-magnetic ion into the system, it shows...

01 July 2022 3,665 5 View

How to determine soluble sugar content in plant materials?

Dear researcher, what is the best, easy and reliable way to test the soluble sugar (i.e., glucose, fructose, sucrose and starch) content and their percentage in plant material? Thank you kind...

17 November 2021 5,488 4 View

Can doping of non-magnetic ion induce spin glass state in a ferromagnetic system?

I have a ferromagnetic DMS system. On doping with a group V element, we find the presence of a spin-glass state along with ferromagnetism. How is this possible?

31 October 2021 8,047 3 View

I got confused when it comes to evaluating the product yield from the GC-MS and GC-FID data. Pls is there a standard formula for evaluating yield?

Many research works presented the yield of their product after reaction using either GC-MS or GC-FID without stating clearly on their manuscript how they arrived on the said values. Pls I need...

09 July 2021 4,111 4 View

What can be known from this ZFC-FC plot ?

I am working on Dilute magnetic semiconducting system and my sample is co-doping of Sb in Fe doped SnTe system. What kind of magnetic phase can be deduced from this plot which shows some kind of...

14 April 2021 9,270 4 View

How antibodies are designed plz explain in step??

from where and how we select sequence of antibody for designing of antibody??i don't understand .kindly tell me.

19 September 2020 2,832 1 View

How to calculate photosynthetic N use efficiency (PNUE)?

hello everyone i had conducted a pot experiment and determined photosynthetic activities, chlorophyll and plant N content. Now i want to calculate photosynthetic N use efficiency (PNUE)in pot...

15 January 2020 4,126 3 View

Can some one share literature on the microbial community in specifically in maize/alfalfa intercropping system?

can some one share literature on the microbial community in specifically in maize/alfalfa inter cropping system?

21 October 2018 8,532 3 View

Why to study rhizospheric microbial community in intercropping system?

hello every one what is the purpose of study rhizospheric microbes? i want to study rhizospheric microbes in cereal/legumes intercropping system but according to my proposal someone ask me why i...

18 October 2018 3,849 6 View

Similar questions and discussions
Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close