Protein kinase, abiotic stress, flooding
I optimized the structure of Pd Acetate Trimer using HF/STO-3G and the B3LYP/LANL2DZ without any solvent. The optimization was successful without any negative frequency. Then I added solvent...
28 September 2020 745 3 View
Can CRISPR-cas9 gene editing technology save patients suffering from low pancreatic beta cell and cure diabetes? If so any human trial going on?
26 June 2020 1,246 4 View
Actually ,i couldnot evaporate DMSO due to high boiling point. So how can i get back my metal complex
01 March 2020 3,212 7 View
I have tree abundance data of six different land use categories and for each category i have taken 12 plots. Now I need to draw Sample rarefaction (Mao tau) curves showing all the six different...
19 September 2019 2,857 2 View
I want to do LC-MS/MS analysis of my plant based samples (Fractions of plant secondary metabolites obtained from column chromatography) . Can someone please suggest to me some good and reliable...
03 March 2019 9,654 15 View
I have deposited thin film of graphene oxide on corning glass and undoped Silicon substrates by dropcast method. I have kept at 50C for 3 hours to dry. Then I have annealed them at high...
03 October 2018 8,794 3 View
I deposited the thin films of MgTiO3 using RF sputtering onto quartz substrate. After XRD analysis of thin films and sputtering target (home made), it is observed that the lattice parameters of...
24 June 2018 4,327 2 View
I have been working on a paper on the role of new media (specially social media) in democracy. My emphasis is on the role of social media in Indian Democracy. But could not able to use a...
16 June 2018 1,848 6 View
I have calculated percentage crystallinity using ( Ac/Ac+Am ) formula for the following curves. But I am getting crystallinity percentage more for the sample C in comparison to A. Am I wrong in my...
21 September 2017 5,629 11 View
What are the substitutes to Sodium azide and EMS as cytotoxic agents?
01 July 2017 6,909 1 View
I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...
01 March 2021 10,078 1 View
Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...
01 March 2021 9,911 3 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...
26 February 2021 3,668 1 View
25 February 2021 6,760 1 View
I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?
25 February 2021 9,761 1 View
