How can the in vitro cytotoxicity data of an extract be extrapolated for evaluation in an in vivo tumor model?
You will require a detailed understanding of the pharmacokinetics and pharmacodynamics of the active substance. The pharmacokinetics must be studied in vivo, measuring the level of the active substance in the plasma and target tissue over time following administration of various doses. It may be possible to study the pharmacodynamics in a simulated in vivo system, such as with a chemostat or hollow fiber system, instead of in vivo.
The concentration of the active substance is of great importance in relation to body weight, in addition to the effectiveness and activity of the substance. Greetings and appreciation
I don't think one can extrapolate in vitro cytotoxicity data into an in-vivo model. what you can do is "paw edema method' if your ultimate goal is selection of a dose.
I think a great deal of understanding of the active ingredients and the extract as a whole may be necessary. Dose optimization and evaluation of relevant parameters in preliminary studies are essential. Extracts can contain many substances with pharmacological importance. Hence, direct extrapolation of in vitro data to in vivo systems would be difficult.
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