In case of testing AFLP for a plant leaves rich in polysaccharides, proteins and phenol, how can protein / phenol contamination of the extracted DNA affect AFLP quality?
Dear all, For discussion: To what extent the ecological change could generate a substantial adaptive evolution on a short time scales (20 to 30 years or within few decades)? with kind...
07 August 2016 3,423 16 View
Recently, many climate indices are known. If one has climate data for a number of years and would like to calculate one of the climate indices, which climate index is achievable with the daily...
03 April 2015 2,819 2 View
Statistically, we use both terms. I would to like to ask on when we say that's a variable may change over time but this is a parameter which is a constant for a certain time or certain conditions,...
02 March 2015 7,373 4 View
Although the role of stem cell is not new, but I would like to discuss what are the added benefits from 1998 till now after the capability of the scientists to remove stem cells from human...
01 February 2015 6,033 1 View
How to write an application for a research project submitted to German Research Foundation (DFG)?
01 February 2015 6,783 1 View
At offering two different sugars as carbon sources, at first microorganisms consume the preferred sugar, then go into a transient growth delay, and finally start in inducing genes to metabolize...
01 February 2015 2,435 0 View
The world’s growing population is now one of the problems affect the human being. I would like to discuss the possible answers for the question: what are we doing to cope with the fast growing of...
01 February 2015 4,337 2 View
I would like to ask on this gene, what its role in causing death? and if anyone knows any way for treatment or prevention the disease induced by this gene?
01 February 2015 1,049 1 View
I would like to ask on what are the main properties used for determining index soil properties for an area? How can one calculate? And what are the factors that affect this index?
01 February 2015 7,384 7 View
I would like to ask on the possibility of transforming the unstable water soluble magnesium-compound (chlorophyll) to the iron-compound (hemoglobin) as the chlorophyll molecule closely resembles...
01 February 2015 1,784 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,983 2 View
Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...
26 July 2024 2,136 0 View