To what extent the ecological change could generate a substantial adaptive evolution on a short time scales (20 to 30 years or within few decades)?

07 August 2016 3,291 16 View

How one can calculate these climate indices?

Recently, many climate indices are known. If one has climate data for a number of years and would like to calculate one of the climate indices, which climate index is achievable with the daily...

03 April 2015 2,652 2 View

What are the differences between variable and parameter?

Statistically, we use both terms. I would to like to ask on when we say that's a variable may change over time but this is a parameter which is a constant for a certain time or certain conditions,...

02 March 2015 7,236 4 View

What are the added roles of stem cell in curing diseases?

Although the role of stem cell is not new, but I would like to discuss what are the added benefits from 1998 till now after the capability of the scientists to remove stem cells from human...

01 February 2015 5,898 1 View

Why microorganisms consume first the preferred sugar then start in inducing genes to metabolize the less preferred sugar?

01 February 2015 2,306 0 View

How to write an application for a research project for DFG?

01 February 2015 6,659 1 View

What is the gene for Gerstmann-Straussler-Scheinker disease or GSS?

I would like to ask on this gene, what its role in causing death? and if anyone knows any way for treatment or prevention the disease induced by this gene?

01 February 2015 837 1 View

What are we doing to cope with the fast growing of the world's population? and What are its predictable impacts?

01 February 2015 4,188 2 View

Is it possible to transform the chlorophyll to hemoglobin outside our bodies?

I would like to ask on the possibility of transforming the unstable water soluble magnesium-compound (chlorophyll) to the iron-compound (hemoglobin) as the chlorophyll molecule closely resembles...

01 February 2015 1,664 4 View

What are the main properties used for determining index soil properties for an area? How can one calculates it?

01 February 2015 7,267 7 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View

How can I improve the concentration of banana fruits extracted RNA?

Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...

22 February 2021 6,126 2 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

Phenol-pH graphic?

We want to use phenol pH graphic to measure the pH of mediums before and after inhibitor application to cells. We have phenol absorbence values at 460, 560 and 720 nm wavelength. When we measure...

20 February 2021 7,700 3 View