14 September 2014 3 5K Report

Suppose that you had run out of Coomassie blue reagent to stain protein in the SDS

-PAGE gel, but still needed to determine how much kinesin - GFP you had in your purified sample. You do, however, have a sample of GFP - tubulin of known concentration, and spectrophotometer available. How could you determine the concentration of kinesin - GFP using the GFP - tubulin and the spectrophotometer? Remember, GFP is green no matter what other protein it is attached to.

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