I have not been able to reproduce experiments in HK2 cells (form ATCC), stimulated with Angiotensin II 1uM, to evaluate fibrosis markers genes. Cells are growing in DMEM:F12 suplemented with 10% Fetal bovine Serum, glutamin, and 5ng EGF. For experiments, 80% confluents cells are FBS depriveted for 24 hours, and then stimulated with Angiotensin II 1uM for 48 hours in media without FBS and EGF. Some advice?
Laura: HK2 cells behave as hepatocytes and require special proteins to support their normal growth. We took advantage of serum-free condition medium for cytokine effects and got good results with WB, qPCR, and ELISA. Check out our paper on my RG account.
Laura Colman Hi,
Did you manage to get results that showed positive stimulation of fibrosis markers?
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