Laura Colman

2 Questions 3 Answers 0 Followers

Questions related from Laura Colman

How can improve HK2 cells to respond angiotensin II stimulus?

I have not been able to reproduce experiments in HK2 cells (form ATCC), stimulated with Angiotensin II 1uM, to evaluate fibrosis markers genes. Cells are growing in DMEM:F12 suplemented with 10%...

19 February 2018 3,873 3 View

Angiogenesis assay using matrigel

I am using growth factor reduced basement membrane extract ( 10mg/ml Matrigel from ATCC) to compare 2 conditioned media (CM) from HUVEC transfected with a siRNA control negative or siRNA against...

26 October 2016 1,240 3 View