Hi all, I intend to assay my cells with L-15 medium supplemented with 17alpha-methyltestosterone.
I dissolved the powdered hormones in 100% ethanol as a stock solution of 100mM. When I add 250ul of this stock solution into 50mL of L-15 mediums they formed small white crystals that will never dissolves. Am I doing something wrong here? Should I use DMSO instead of ethanol?
regards,