Could anyone share a protocol for the isolation of Sarcoplasmic reticulum from mice muscle ?

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Gordon L Warren

What do you want to do with the sarcoplasmic reticulum? I am attaching the SR isolation procedure we have used when wanting to perform calcium release and uptake assays (see p. 59). Our prep might be considered a crude prep by others. However, it worked fine for us.

Benudhara Pati

@Gordon L Warren.. thank you

Rizwan Qaisar

We used it for SR calcium uptake assay. I have attached the protocol (SR purification and SERCA activity) and the paper where we published it.. wishes