Hi everyone!

I would like to do live cell imaging of HEK293T cells on 8-well IBIDI glass bottom chambers. However, when I seed the cells, they always accumulate at the center (and some along the edges, but nothing in between). I already tried shaking the plates better after seeding (though the volume is so small, nothing really moves); and I tried coating the wells with poly-d-lysine first, but all in vain.

Does anyone have a suggestion how to make the cells cover the chamber bottom evenly?

Best,

Nina