For extraction of non polar fractions, non polar solvents are used. Usually hexane, or petroleum ether. You could mix lyophilized sample with non polar solvent, sonicate/vortex mixture, centrifuge, and separate liquid from solid phases (procedure usually is repeated two or three times, extracts are combined, and solvents evaporated under vacuum and nitrogen atmosphere). Or if you want to fractionate alcohol extract, you could mix your ethanol extract with hexane, or other suitable non polar solvent, vortex mixture, and separate two phases.
thanks a lot Angel. I have hexane fraction and i want to use it in cell culture media, so I couldn't find a desired solvent that solve my fraction and not be toxic for cells.
Sorry, I didn't understand the question. I thought, it is about extraction of nonpolar fraction from cell culture, not in. My mistake.
I dont have experience with cell biology, but can I give you one unconventional idea? You could use phospholipids to make artificiall liposomes, that could incorporate hydrophobic fraction. I used 10mMol/l TRIS-HCl buffered (pH7.4) saline solution with 5mg/ml egg phospholipide, to analyse hydrophobic fractions in few studies. I think modification of this method should work for cell cultures too.
thank you so much Angel, I ll try to use phospholipids and hope to be effective.
- Badges
- Science topic