Which subunit is the best one to measure for assaying NF-kB activation via Real time-PCR?

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Isehaq Al-Huseini

Dear Fereshteh Jeivad

You can measure the activity of NF-kB by checking expression of I kappa B-alpha mRNA using RT-PCR

Article Monitoring NF-κB Transactivation Potential Via Real-Time PCR...

Or by western blot you can check phospho-P65

Hope this would be useful.

Best regards

Isehaq

Thomson Patrick Joseph

Hello,

I would suggest you directly go for NF-kB activity assay or you can do western blotting for phospho-p65.

Cheeers.....