How can I separate two DNA fragments of 3000bp and 3700bp using polyacrylamide gel electrophoresis?

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Hanna Alalam

Why would you separate fragments of this size using polyacrylamide gel electrophoresis? standard 0.8%-1% agarose gels can resolve these fragments.

Robert Adolf Brinzer

Use a large gel and let it run to the bottom (can take most of the day).

Lewis Hun

You can also try higher agarose gels % 1.5 to 2% will do it.

Ivan Slovokhotov

Just try agarose gel, about 1,5%, low voltage for a long time. It should resolve clearly