Lewis Hun

3 Questions 15 Answers 0 Followers

Questions related from Lewis Hun

Can someone help me with zika virus cells culture protocol?

Hi everyone, I have experience growing dengue virus with c6/c36 cell line. However, I don’t know whether or not there is a different when it comes to growing Zika. If you have any experience or...

12 April 2016 5,970 6 View

What are techniques to detect protein without specific antibodies?

Hi everyone, I am trying to detect a very small protein (around 8 kda) and I found that my antibodies is not working (after repeated 4 times with various concentration) for this protein....

24 February 2015 5,753 10 View

Does anyone have experience with Protein Expression and Purification?

Hi, I am new to this topic. I constructed a recombinant protein with 6 His tag at the N-terminal. The expression host is BL21 (DE3)plysS. I checked the sequence multiple times and everything is in...

21 August 2014 7,170 20 View