Lewis Hun

5 Questions 20 Answers 0 Followers

Questions related from Lewis Hun

SuperOxide Dismutase Help!

I am using the 19160 SOD Assay Kit-WST (Sigmma) for my SOD experiment between young flies and old flies. I found that young flies has % inhibition of 60, while old flies has % inhibition of 30....

27 May 2017 7,335 2 View

Can someone help me with zika virus cells culture protocol?

Hi everyone, I have experience growing dengue virus with c6/c36 cell line. However, I don’t know whether or not there is a different when it comes to growing Zika. If you have any experience or...

12 April 2016 6,019 6 View

What are the techniques that can be used to determine specific gene function?

I have this scenario where I know that increased of A-pathway lead to longer live with induction of gene-B. I want to design an experiment to prove the longer live is due to gene-B. What...

06 April 2015 2,113 3 View

What are techniques to detect protein without specific antibodies?

Hi everyone, I am trying to detect a very small protein (around 8 kda) and I found that my antibodies is not working (after repeated 4 times with various concentration) for this protein....

24 February 2015 5,803 10 View

Does anyone have experience with Protein Expression and Purification?

Hi, I am new to this topic. I constructed a recombinant protein with 6 His tag at the N-terminal. The expression host is BL21 (DE3)plysS. I checked the sequence multiple times and everything is in...

21 August 2014 7,213 20 View