Solar energy production forecasting ?

Hello everyone! I am interested in the topic of solar energy production forecasting for a ground-mounted PV system of more than 100MW capacity. Can anyone please suggest what are the main data...

08 February 2021 6,834 5 View

Why AC cables are always covered with the sand first and then covered with soil in solar park ?

I was curious to know why we only use sand for covering the AC cables inside the trenches and instead of having good isolation why these cables are covered. Why is there a problem in the trenches...

13 January 2021 6,008 3 View

Error encountered in executing MCE ?

In Terrset, an error encountered in executing Spatial Decision Modeller (MCE). During execution or run the MCE a massage come on the screen that reflects "Your columns and rows are not same" , I...

07 December 2020 1,542 1 View

How to do extract Urban Heat Island separately from LANDSAT image for each land classes?

I have already been read many articles and found some methods but those are not cleared to me. Can you please suggest me how to generate different LST map of each land classes using ArcGIS? Or...

25 October 2020 5,978 5 View

Can someone advise me to do Rietveld Refinement of the material whose Crystallographic Information Framework (CIF) file is not available?

10 October 2020 7,411 9 View

Recombination in Light emitting diode?

Why does recombination release light? It's one thing to say, 'and when the electron drops down it releases light', but it would be another level to explain how an electron dropping down creates...

28 July 2020 2,360 4 View

Has this COVID19 pandemic affected the renewable energy sector ? If yes can we analyse this affects with the help of data science and how ?

As the pandemic has affected each and every sector of the market, so my question is how big it has affected the renewable energy sector. If it has affected then can we have the specific results...

25 May 2020 6,643 7 View

No title

1. Why both models ("cv_mars" and "mars1") produced the same results? What is missing here? Even used another hyper_grid as Please click here https://stackoverflow.com/q/61216514/12675265

27 April 2020 3,876 1 View

How to conduct Monte Carlo uncertainty analysis for actual and predicted data in R ??????

I would like to conduct Monte Carlo uncertainty analysis for actual and predicted data in R; Kindly share some effective resources; However, I have this where understanding the hyperparameters...

08 April 2020 458 2 View

Which model can be used for a mix of I(1) and I(2)?

Dear members, I have a set of five time series. Two of them including dependent variable are I(2) and rest are I(1). Suggest me which time series modelling can be applied in this case? Thank you...

25 January 2020 2,780 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

What lysis buffer is compatible with Bradford assay?

Hello! I am planning to use Bradford assay to measure protein content in PBMCs lysates. The problem is that Bradford assay doesn't seem to be compatible with Tris-based buffers or SDS. Are there...

10 February 2021 1,797 5 View

Macrophages in culture not growing/ dying or detaching . Why ?

Hello, I am an undergraduate student doing my dissertation, where I isolate PBMCs from leucocyte cones in order to plate monocytes and allow for macrophage differentiation. Although the numbers...

09 February 2021 5,334 8 View

Ferret PBMC Fc block?

Hi all, I'm going to run Flow Cytometery on ferret PBMCs but can't find an Fc block for ferret PBMCs. Does that even exist? Do I need ferret-specific Fc blocking antibodies or can I use:...

18 January 2021 4,504 5 View

Minimal protein concentration from BCA can be detected using western blot?

hello, i extracted protein from PBMC's and after applying BCA assay on 2 samples the protein concentration was 400 ug/ml which means 0.4 ug/ul so does western blot work with these quantities? any...

24 December 2020 6,521 3 View

What are possible troubleshooting methods for smearing obtained from PCR products?

I have been trying to amplify DNA isolated from PBMCs. However, after PCR on running samples on AGE, I am getting smears on my gel. I have been using 1% gel and TAE buffer.

18 December 2020 526 2 View

Protocol for activating PBMCs using PHA/IL-2?

Hi there, just looking for a simple protocol stimulating 1 x 10^6 PBMCs/ mL in RMPI + 10%FBS using PHA and IL-2? I am looking for CD25, NKp44, c-kit expression and a few other markers for flow...

08 December 2020 2,087 1 View

How to evaluate human peripheral blood B cell activation?

I want to evaluate B cell activation by flow cytometry using B cells in human peripheral blood. I have difficulty in establishing a positive control. What is the best stimulator?...

27 November 2020 2,996 2 View