Impaired kinesthesia is common in peripheral neuropathies. We want to know various objective tools by which was can asses imapired kinesthesia. Quantification of kinesthesia is our aim to asses the recovery of impaired kinesthesia.
Generally, the target material is Mo, Co, Cu, Fe, Cr, etc, so on what basis should we choose this material?
31 July 2024 5,824 0 View
There is rapid surge of dengue cases in Southern parts of India. However there are no cases till now in the setting I work in Northern India. Besides dengue surveillance and local preventive...
14 July 2024 2,935 2 View
I have read some papers but i didn't get proper answers. In some cases, my level of theory is not exactly written. I will be very gladfull for any recommendation
10 June 2024 9,929 1 View
I am using the liquid electrolyte EC:DMMP(50:50 wt%) mixed upto 1M LiTFS. Usually, the electrolyte obtained was colorless ...but the Last two times, it gave a yellowish color. Why?
01 June 2024 8,355 1 View
I have intel iris xe graphic card. i want to run dft calculation in gaussian using a gpu. what are the necessary modifications to the input file? my input file read like...
21 May 2024 3,402 6 View
If we get a unsatisfactory water report from water supply from a locality? What preventive measures should be taken in already scarce water situation?
27 April 2024 851 0 View
I have collected data from the field in consecutive days. I have those data in excel how can i calculate the exact day of 75% seed germination using any formula.
17 April 2024 1,077 3 View
We are witnessing silent epidemic of Vitamin D deficiency in India. Any solution to problem of RO water and loss of minerals?
10 April 2024 4,716 0 View
We are witnessing rising incidence of mumps . Adults are also getting complicated mumps in form of orchitis. Any reasons to explain?
07 April 2024 8,983 0 View
while trying to generate CGenFF topology for Ligand after extracting from docked file, i got error which i'm showing as a picture. What could be the possible reason and how will i rectify it for...
26 March 2024 2,491 3 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...
24 July 2024 2,158 2 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View
Hi, I need help in detaching adherent monocytes from well plates. I isolated the PBMCs from buffy coat and I did CD14+ positive selection to collect only the monocytes. I let them adhere on my...
20 July 2024 7,922 2 View
I am currently designing a study involving the examination of changes in RNA profiling of PBMCs before and after exercise. To achieve this, we need to collect whole blood samples before and after...
03 July 2024 2,891 1 View
I'm planning on activating 5 million PBMCs with CD3/CD28 at the same time applying a treatment, then incubate for 72 hours. Then I seperate cell subtypes using antibody sequencial seperation...
01 July 2024 8,892 3 View
Hi everyone, I am trying to induce foam cells in macrophage differentiated from PBMC. Which type of staining do you prefer to use? Nile Red or Oil RED O ? What concentration and timing of ox-LDL...
01 July 2024 8,286 0 View
Image attached is at 20X. Cultured media for checking presence of bacteria and yeast and both tests were negative.
27 June 2024 7,473 4 View
I'm trying to obtain MSCs from whole blood PBMCs and from the CD34- fraction of umbilical cord blood. However, I'm obtaining other types of cells that prevent the MSCs from growing. These cells...
26 June 2024 3,664 1 View