Door stuck of StepOnePlus RTPCR System?

I was trying to conduct an experiment on StepOnePlus RealTime PCR System (Applied Biosystems) using qPCR Tubes supplied with the machine, but the door got stuck and won't come down even after 4...

11 April 2019 9,643 2 View

What are the 'must take' precautions for shade drying Algal biomass?

I collected the algae from the shore, removed any impurities and shells they were attached, let them hang in shade to remove excess water and spread them in shade to dry. Later i wish to powder...

11 February 2018 8,802 2 View

What is the specific use of normal and heat inactivated FBS in cell culture?

I do know that the heat inactivation will kill all the complement protiens and some cell lines may or may not grow better in inactivated FBS. How important is it? and how to know wether i need an...

11 February 2018 6,748 13 View

I wish to stain sections of algae (Specifically: order Ceramiales). Can anyone suggest an alternate stain for Aniline blue?

Centroceras and Ceramium are mostly stained using aniline blue.

19 January 2016 3,759 3 View

Any advice on equivalency between two different analytical methods (in-house and compendial method)?

03 December 2015 1,412 3 View

How do I make direction dependent cohesion and friction angle using Drucker-prager constitutive model for anisotropic soil in Abaqus?

How we can assign cohesion and friction angle using Drucker-prager model for anisotropic behavior of soil in abaqus ?

12 November 2015 7,721 1 View

How can I plot deviatoric stress vs. axial strain of whole model in triaxial test from odb field output in Abaqus?

Thanks in advance for your replies.

08 April 2015 1,644 7 View

DNA shearing in gel electrophoresis, suggestions?

15 July 2014 4,215 9 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

How to model the battery pack in PSIM or PSCAD?

Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...

28 February 2021 2,116 1 View

How to find the right number of integration points in second order 3d finite elements?

I am familiar with finite element simulations and numerical schemes like the Gauss Legendre quadrature. I also know that the Gauss integration yields exact values for polynomials up to degree 2n-1...

27 February 2021 6,818 3 View

High aspect ratio hexahedral mesh optimization solver?

I am interested in evaluating mesh optimization solvers that converge to high aspect ratio unstructured hexahedral grids. In other words, I am interested in a solver that optimizes warpage,...

26 February 2021 7,279 2 View

How does an AC-DC power supply Flyback Converter Based USB Charger Model using Matlab/Simulink looks like?

I am required to learn about Flyback converters and I got stuck not knowing to full design of the power supply flyback converter Based USB Charger Model using Simulink, especially the design model...

25 February 2021 5,435 2 View

Sample size determination for non-parametric tests?

How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...

24 February 2021 166 6 View

Anyone with some Transgenomic Wavemaker software? Seeking help from the chromatography community?

I am trying to seek out some Transgenomic Wavemaker software (happy to purchase a computer too) to operate a fraction collector. The only way to connect to the fraction collector so far is to...

23 February 2021 9,181 1 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View