We used Zeiss LSM880 & Zen software for tiling images, scanning approximately 32 areas in one tile image. How can I save these 32 images individually, not as a tile image? Attached is an example for the DAPI channel.
I would recommend you use ImageJ or FiJi to extract each vignette and save them. This will need some programming but it's fairly straightforward. I did it some years ago : extracted each single pictures from the montage picture and produced a stack from them.
Try the attached ImageJ macro and adapt it if needs be