Hanadi Marzouq

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Questions related from Hanadi Marzouq

FCCP induced Hyperpolarization of MMP?

Hi, I used the JC10 kit (MAK159) to measure mitochondrial membrane potential in neuronal cells. For positive controls, we used FCCP at 10 µM and oligomycin at 3 µg/mL, with the former to induce...

16 December 2024 1,906 0 View

Merge Intensity Data from 2 runs?

Hi, We have collected several images for two independent runs of an experiment using two different confocal microscopes and settings. Thus, the intensity values collected through ImageJ were...

23 July 2024 8,869 0 View

Why I have this weird green signal in the nucleus?

Hi, In a few wells and cells, we’ve noticed an unusual signal in the nucleus using both eppifluroscence & confocal microscopes. Does anyone know why this is happening? It occurred twice in...

11 July 2024 2,195 0 View

How can I separate 1 tile image into separate images?

Hello, We used Zeiss LSM880 & Zen software for tiling images, scanning approximately 32 areas in one tile image. How can I save these 32 images individually, not as a tile image? Attached is...

12 March 2024 830 2 View

Why I had empty wells in 7 out of 8 after performing Immunostainong protocol?

I followed an immunofluorescence staining protocol for my cells differentiated into neurons at D14. Using 4% PFA (Alfa Aesar product) for fixation in PBS, I observed that 2-3 out of 8 wells were...

03 February 2024 8,981 10 View

How can one quantify the number of particular cells exhibiting a signal within FL images out of the total cell population using Fiji/ImageJ?

Hello, We conducted three stains for Nucleus, Outer membrane, and a particular phosphorylated protein expressed in a subset of cells. After capturing random images, our focus is on enumerating...

02 February 2024 6,126 1 View