Hello everyone,
To examine oxidative stress, inflammation and apoptosis parameters in the brain tissue of a Parkinson's model rat, should i select specific regions in the brain or can i use total brain tissue homogenate?
I'm open to any kind of advice.
Thank you in advanve.
Hello Naile Merve Guven,
Parkinson's disease is a progressive disorder that is caused by degeneration of nerve cells in that part of the brain called the substantia nigra, which controls movement. These nerve cells die or become impaired, losing the ability to produce an important chemical called dopamine.
The lack of dopamine creates physical symptoms of the disease, particularly motor symptoms such as tremors and slowed movement. It’s a gradual progression, and at the time of diagnosis, typically patients would have already lost over half of their dopamine-producing neurons.
I would suggest you focus on substantia nigra.
I have attached a few references that may help.
https://pubmed.ncbi.nlm.nih.gov/12469866/
https://www.en-journal.org/journal/view.html?uid=167
https://pubmed.ncbi.nlm.nih.gov/18343932/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5964493/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8948331/
https://www.imrpress.com/journal/JIN/18/4/10.31083/j.jin.2019.04.192/htm
Best.
Hi, Naile Merve Güven
Analysis of brain regions such as the substantia nigra, striatum, brainstem nuclei, cortex, and hippocampus is commonly conducted in rat models of PD. These areas are crucial because they show the neuroinflammatory reactions, changed neurotransmitter levels, and neuronal degeneration typical of PD. Examining these domains facilitates comprehension of the disease's dopaminergic neuron degeneration, protein aggregation, and cognitive impairments. While utilizing total brain tissue homogenate offers a more comprehensive evaluation, focusing on particular brain regions enables a more precise investigation. Ultimately, the decision is based on your study’s goals, the resources you have at your disposal, and the amount of detail needed.
Article Cerebral and cerebellar histological changes in the rat anim...
Article Neuroprotective effects of curcumin on the cerebellum in a r...
Good luck!
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Biochemical Techniques
- Analytical Chemistry
- pH