15 Questions 4 Answers 0 Followers
Questions related from Naile Merve Güven
Is there a protein concentration range available for cell culture supernatant? (e.g. supernatant from HeLa cells) could you help me out?
30 January 2025 9,793 2 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
30 July 2024 4,720 1 View
When evaluating the expression of superoxide dismutase, catalase and glutathione peroxidase enzymes by ELISA in cell culture studies, should I use the supernatant or lysate part of the...
25 March 2024 5,308 2 View
in the Parkinson's model created with 6-OHDA, the application is performed unilaterally intrastriatically. after tretment, should the dopamine level be measured through the striatum on the side...
25 March 2024 9,360 3 View
Hello everyone, To examine oxidative stress, inflammation and apoptosis parameters in the brain tissue of a Parkinson's model rat, should i select specific regions in the brain or can i use total...
15 March 2024 2,370 2 View
Hello everyone! MTT was performed after applying DEHP to HeLa cells. According to MTT results (24 hours of exposure), cell viability is significantly reduced at concentrations of 100 micromolar...
28 February 2024 568 2 View
Hello everyone! I have never worked with glucose-6-phosphate dehydrogenase in powder form. In which solvent should I dissolve G6P-D (https://www.sigmaaldrich.com/TR/en/product/sigma/g6378) in...
13 February 2024 5,841 0 View
Hello everyone! I measured the protein concentration by BCA for each well of HeLa cells seeded in 6-well plate (750000 cells / 2ml DMEM per well). Protein concentration for each well was...
07 February 2024 7,572 2 View
hello everyone! HeLa cells were seeded in 6-well plates at 200,000 cells per well. They were left for 2–3 days to reach 70–80% confluency. Then, determination of protein concentration was...
01 February 2024 2,558 5 View
Hello everyone, After exposing HeLa cells to phthalate, I will detect various antioxidant levels with an ELISA kit. Before ELISA, I will determine the protein concentration of the cells with BCA...
26 January 2024 812 1 View
hello everyone my samples are HeLa cells. i‘ll use them for ELISA assay. before elisa, i’ll perform BCA assay. during bca, i’ll dilute my samples. what do you recommend for dilution solution and...
24 January 2024 7,810 3 View
Hello everyone, I will work with HeLa cells and expose them to di-2-ethylhexylx phthalate (DEHP). Then, i will perform ELISA. Is it necessary to determination of protein concentration before...
23 January 2024 4,876 1 View
i want to homogenize pancreas tissue to perform antioxidant enzyme activity. how should i prepare homogenization solution?
06 October 2023 1,779 0 View
I will work with HeLa cells and expose them to di-2-ethylhexylx phthalate (DEHP). I've been reading articles to choose DEHP doses to try in the MTT test. Is there any different guideline or...
20 September 2023 6,860 3 View
MTT assay was performed in HeLa cells for di-2-ethylhexyl phthalate in the micromolar working range of 1-3-10-30-100-300-1000-3000. According to the results, while cell viability decreased up to...
01 January 1970 3,047 0 View
