06 October 2023 0 2K Report

i want to homogenize pancreas tissue to perform antioxidant enzyme activity. how should i prepare homogenization solution?

More Naile Merve Güven's questions See All
EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Should I use the supernatant or lysate part of the cell?

When evaluating the expression of superoxide dismutase, catalase and glutathione peroxidase enzymes by ELISA in cell culture studies, should I use the supernatant or lysate part of the...

24 March 2024 5,247 2 View

How can i select proper part of brain tissue?

in the Parkinson's model created with 6-OHDA, the application is performed unilaterally intrastriatically. after tretment, should the dopamine level be measured through the striatum on the side...

24 March 2024 9,292 3 View

How can i select proper part of brain tissue?

Hello everyone, To examine oxidative stress, inflammation and apoptosis parameters in the brain tissue of a Parkinson's model rat, should i select specific regions in the brain or can i use total...

14 March 2024 2,305 2 View

Creating oxidative stress model in cell culture?

Hello everyone! MTT was performed after applying DEHP to HeLa cells. According to MTT results (24 hours of exposure), cell viability is significantly reduced at concentrations of 100 micromolar...

27 February 2024 503 2 View

Relationship between protein content and cytotoxicity results?

Hello everyone! I measured the protein concentration by BCA for each well of HeLa cells seeded in 6-well plate (750000 cells / 2ml DMEM per well). Protein concentration for each well was...

06 February 2024 7,499 2 View

Protein concentration of HeLa cells?

hello everyone! HeLa cells were seeded in 6-well plates at 200,000 cells per well. They were left for 2–3 days to reach 70–80% confluency. Then, determination of protein concentration was...

31 January 2024 2,495 5 View

Determination of HeLa cells protein concentration via BCA?

Hello everyone, After exposing HeLa cells to phthalate, I will detect various antioxidant levels with an ELISA kit. Before ELISA, I will determine the protein concentration of the cells with BCA...

25 January 2024 754 1 View

Protein dilution for BCA assay?

hello everyone my samples are HeLa cells. i‘ll use them for ELISA assay. before elisa, i’ll perform BCA assay. during bca, i’ll dilute my samples. what do you recommend for dilution solution and...

23 January 2024 7,747 3 View

Determination of protein concentration before elisa?

Hello everyone, I will work with HeLa cells and expose them to di-2-ethylhexylx phthalate (DEHP). Then, i will perform ELISA. Is it necessary to determination of protein concentration before...

22 January 2024 4,816 1 View

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While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

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Ideal tissue thickness for multiple immunolabelling and confocal microscopy?

Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...

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Why is this tissue slice different?

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