I want to isolate adipocytes from human adipose tissue. but after isolation and culture them, there were very larg droplets between small to moderate size cells in 12-wells plate that seemingly they are oil layer, not adipocytes. I want to have pure adipocytes without oil. Already in research gate someone recommended to use syringe during isolation to remove oil. It did not work for me. The layers can very easily mix together. What do you recommend?
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