I want to adhere mature human adipocytes to bottom of Petri dishes or flasks for cell culture. Which coated material or method do you recommend?

07 March 2024 1 776 Report

After mature adipocytes isolation from human adipose tissue, I want to adhere them for my studies (Adipocytes tend to suspend on top layer of the media). Which method or materials do you recommend for coating (with a minimum effects of adipocytes differentiation, shapes and viability)? Poly-L-Lysin? Collagen-coating? etc. Thank you so much for your guidance.

Malcolm Nobre

Hello Amirhooman Asadi

I would recommend collagen.

Collagen is the main ECM component and contributes considerably to the non-cell mass of the adipose tissue. Collagen is primarily produced by the adipocytes. Moreover, collagen contributes to cell adhesion, migration, differentiation, morphogenesis, and wound healing in the adipose tissue. Between the collagens, collagen IV is a major component in each adipocyte as basement membrane, and this is necessary for adipocyte survival.

You may want to refer to the articles attached below for more information.

Article Extracellular Matrix Remodeling of Adipose Tissue in Obesity...

Article Adipose Extracellular Matrix Remodelling in Obesity and Insu...

Article Adipocyte extracellular matrix composition, dynamics and rol...

Best.

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