Amirhooman Asadi

5 Questions 1 Answers 0 Followers

Questions related from Amirhooman Asadi

Is there any protocol about MTT assay for human mature adipocytes ?

Most of the MTT assay protocol are for preadipocytes or cell lines, not isolated human mature adipocytes .I am looking for Suitable plate, Seeding cell number, suitable stop reagent, incubation...

13 September 2024 5,870 1 View

What is the suitable freezing media and protocol for human mature adipocytes cryopreservation?

I used regular freezing media (90% FBS and 10% DMSO) for cryopreservation of isolated human mature adipocytes from adipose tissue in -80 degree. When I freezed the cells, the number of cell was...

27 May 2024 8,026 0 View

How it is possible to analyze human mature adipocytes cell markers with flow cytometry? what are the essential optimizations?

As human adipocytes can be up to 300 μm in diameter, How these cells can be analyzed for cell markers by flow cytometry with 100 micron Nozzles? What changes are necessary to be done on flow...

13 May 2024 2,293 0 View

How can i remove oil layer from adipocyte layer in human mature adipocyte isolation?

I want to isolate adipocytes from human adipose tissue. but after isolation and culture them, there were very larg droplets between small to moderate size cells in 12-wells plate that seemingly...

21 March 2024 5,995 0 View

I want to adhere mature human adipocytes to bottom of Petri dishes or flasks for cell culture. Which coated material or method do you recommend?

After mature adipocytes isolation from human adipose tissue, I want to adhere them for my studies (Adipocytes tend to suspend on top layer of the media). Which method or materials do you...

07 March 2024 956 1 View