Hello everyone, in the gel results image provided, the samples in lanes 2 and 5 represent linearized plasmid DNA from groups A and B respectively. Additionally, the samples in lanes 3 and 6 depict circular plasmid DNA from groups A and B respectively. Nothing was loaded to Lane 4. I am curious as to why there seems to be leakage in the sample of lane 6. Also, do you think this leakage occurred immediately after loading or during the gel run? Moreover, could the anomaly observed in lane 4 be linked to the leakage from the sample in lane 6?
I'd appreciate all your inputs.
This does not look like leakage but rather that a fiber or something similar is embedded in your agarose gel and/or that you did not properly mix in the gel staining dye before pouring the gel. Make sure that your buffers are not contaminated with anything and that you properly stir in the dye before casting the gel.
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