How can I remove Epithelial Sheet from a culture dish?

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Yoshitaka Kawato

There are some special culture dish which are commercially available.

Please check the following website.

http://www.cellseed.com/product-e/004_caution.html

Richard von Furstenberg

The dishes Yoshitaka posted look neat. If you have some cells to experiment with you may want to try 3mM EDTA in PBS, incubate at room temp for a bit (10-20 min or so), and see if the sheet starts to release from the plastic.

Editing to add that I've never tried this :) But I have used concentrations of 3-5mM EDTA to remove intestinal epithelium in large sheet-like pieces from the intestinal basement membrane..

Sheida Jamalzadeh

Yoshitaka, tnx for the reply. I need to culture my cells on a gel. I wonder if this membrane is able to harvest the cell sheets from the gel as well or not.

Richard,

Tnx for the reply. How about the EDTA, is that also good for removing the cells from the gel ?

Oh, are your cells in a monolayer on top of collagen or something similar?

Hi Sheida,

Do you mean that you harvest the cells on gel-coated culture dish?If so, I recommend you to purchase the special dish which the Cellseed company sells and you coat with gel.

Or, after you finish culturing cells on gel, use `Cellshifter` which is the tool to use for separate the cell from gel. And they are also dealed in Cellseed company.

They are speciallized company on regenerational medicine, if you have any question, I recommend you to inquire them.

Makoto Kondo

What specific type of cells are you using?

If keratinocyte (stratified epithelium), you can easily lift up the confluent cells using UpCell with fine forceps.

I need to culture liver cell lines on top of a gel (collagen/matrigel), after that need to harvest the cell sheet from top of the gel to transfer it to another dish for more treatment.