Defining mixed model with repeated measures data?

HI all, I have been working with daily diaries data ( 6 emotions of which 3 positive and 3 negative) were assessed for 30 days. These observations (1level data) are then nested within subjects...

13 February 2021 1,810 3 View

High percentage agarose gel for better resolution?

Hi all, it might be a very basic question, but I need some opinions: I need to separate a 294 bp wild type PCR product from a 232 bp knock-out PCR product. I want to do DNA extraction out of the...

08 June 2020 9,557 9 View

What do you think of the impact of Covid-19 on faster acceptance of innovative healthcare technologies?

After this pandemic outbreak, nothing will be the same. Previously suspicious attitude toward accepting innovative solutions in clinical practice and long times for regulatory procedures and...

09 April 2020 7,691 10 View

Any experience with Health Informatics Journal?

We would like to publish something which is in the domain of Health Informatics, but several Journals have almost identical names. any suggestions, experience with publishing with those Journals...

22 February 2020 7,635 9 View

Do we need a standardization prior to publication of any machine learning application?

What do you think about so high accuracies reported in so many publications especially in medicine and psychiatry? Talking about overfitting and so frequent lack of internal and external...

07 October 2019 2,558 4 View

Is it possible to stain cells with Acridine orange/ethidium bromide in hydrogel 3D invasion assay?

I am planning to perform 3D invasion assay with spheroids in hydrogel and to visualise live/dead cells. Is it possible to visualise cells with AO/EB (besides CAM/EtHD) dyes? Thanks

18 October 2018 5,722 3 View

Can LPS be captivated by the tube if you freeze and subsequently thaw it?

15 January 2017 7,051 3 View

Does someone can recommend a good Elisa kit for mouse NTx (N-terminal telopeptide ) ?

Hello everyone, I need to measure NTx (N-terminal telopeptide) marker in a mouse serum. I would really appreciate if someone could share experience with this kind of ELISA kit, most importantly...

10 April 2016 5,458 1 View

What is wrong with my intestinal organoids?

I am trying to grow swine intestinal organoids from crypt preps. I have been doing it with mouse tissue for at least a year now. For the pigs, a few days after getting the crypts, there are...

05 April 2016 3,294 7 View

What is wrong with my intestinal organoids?

I am trying to grow swine intestinal organoids from crypt preps. I have been doing it with mouse tissue for at least a year now. For the pigs, a few days after getting the crypts, there are...

03 April 2016 8,579 8 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

What software programs can directly simulate Additive Manufacturing Directed Energy Deposition (DED)?

I understand there are a lot of software programs that can simulate DED by going through specific modeling, simulation, and commands. For me, that was time consuming and results were not accurate....

24 February 2021 4,428 3 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

Which programs are to be used to register 2D image onto a 3D image data set?

I am trying to register a light micrograph onto a 3D CT data set, as I want to extract the corresponding image from the CT data set. Is there any software or program can do this accurately? I am...

21 February 2021 8,220 3 View

What is the better software for approximation of 3D surface with equations?

I have a massive of x, y, z coordinates of 2D surface in 3D space, which builded in Ansys HFSS or AutoCAD. How can I get the analitical aproximation of this surface by formulas?

21 February 2021 3,444 7 View

How can I obtain Reaction Forces in ABAQUS?

I am trying to simulate a simplified version of a beam resting on 10 points using Abaqus. The solutions I want to obtain are the Reaction Forces in each resting point or area when applying a...

21 February 2021 3,075 2 View

How to convert 2D stack images to 3D to obtain surface area of nucleus?

I have 15 slices of the nucleus at different z-heights from confocal imaging. I would like to use these slices to reconstruct the 3D nucleus and ultimately estimate the surface area of the...

20 February 2021 6,967 1 View

How to get the vector of a specific vibration mode in phonon dispersion?

For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...

20 February 2021 4,332 4 View

Has anyone used deep learning for 2D/3D REGISTRATION ?

I am a Master student in Biomedical (Bio-electrical) Engineering and work on " 2D-3D registration ( CT and X-Ray) as my thesis. I created DRR from CT and want use CNN( Convolutional Neural...

19 February 2021 6,354 3 View

PV system design. 3d modelling and Analysis?

I have been looking for a good cost-effective tool for 3D modelling a glass-glass solar module system. I was initially considering Skelion as a tool but it does not look very promising, a bit...

13 February 2021 8,126 1 View