Milena Korneck

6 Questions 8 Answers 0 Followers

Questions related from Milena Korneck

How can I purify membrane-bound proteins in neuronal cell culture?

Hi all, I need to do a western blot of a protein where one isoform is ER-membrane-bound. My antibodies do not show that isoform reliably and someone suggested it could be lost due to my RIPA...

09 November 2023 6,972 0 View

Why can't I choose the "Find cytoplasm" command in columbus (perkin elmer)?

Hey everybody, I recently started analyzing live cells in the Operetta system. They are stained with Tubulin tracker (633) and Mito tracker (546) and no nuclei stain since I want to keep them...

19 July 2023 4,057 1 View

How long can you keep fixed cells in the fridge?

Hi all, I have to postpone some stainings and imaging due to technical difficulties, but the cells are already in culture. They are hiPSC derived cortical neurons in 96well plates and the staining...

23 March 2023 4,613 3 View

How to resolve aggregating antibodies?

I've had issues with my secondary antibodies for ICC. They seem to form complexes which then result in bright artificial spots in the images which I cannot attribute to protein complexes rather...

10 May 2022 2,523 1 View

High percentage agarose gel for better resolution?

Hi all, it might be a very basic question, but I need some opinions: I need to separate a 294 bp wild type PCR product from a 232 bp knock-out PCR product. I want to do DNA extraction out of the...

09 June 2020 9,731 9 View

Data organization in research

Recently, I heard about DataLad to organize and reproduce your data. Does anyone here use this? How does it work out for you? Or else, what means do you use to organize yourselves and your data? I...

01 January 1970 1,424 0 View