I am using hexane sulfonic acid as ion pair reagent. pH is 3.85. Mobile phase is Buffer and methanol 35:65 ratio. seperated two analytes in this method. C18 column with end cap i am using. How can I improve the shape of these peaks?
if added TEA in mobile phase one analyte peak is eluted at void volume. please any one can suggest other than TEA, posible to reduce the peak tailing
Did you compared the tailing for different pH? What "buffer" you use?
In order to answer your question properly, you should give us your molecules of interest, your chromatographic conditions, and post an example chromatogram. Peak tailing or fronting can have many reasons but in general the molecules are 'not happy' in their environment (mobile phase and column).
What are your analytes? We have used both TEA and DMHA as ion pairing reagents for various purposes.
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