Therapeutic intervention (agonist) increases total protein level, however, phosphorylation is decreased (neutralized by total protein). What would be the possible reason?
If anyone has any paper related to this please share.
There are several scenarios where phosphorylation may decrease while the total protein level increases:
1. Regulatory Mechanisms:
- Phosphorylation acts as a reversible modification that can switch a protein between active and inactive states. The dephosphorylation of a protein can deactivate it, even if the total amount of that protein in the cell has increased.
2. Cellular Signaling Pathways:
- Upregulation of protein expression (increase in total protein level) may occur as part of a cellular response to external signals or stress. However, the phosphorylation status of the protein may be regulated independently of its expression level.
3. Feedback Inhibition:
- In certain signaling pathways, an increase in the total protein level may trigger feedback inhibition mechanisms. This can involve dephosphorylation of the protein to dampen the signaling response, even though the protein's overall abundance has increased.
4. Cell Cycle Regulation:
- During the cell cycle, various proteins involved in cell division are regulated by phosphorylation. After a cell divides, some proteins may be dephosphorylated to prepare for the next round of cell division, even if their total levels have increased.
5. Protein Turnover:
- Increased synthesis of a protein may be accompanied by increased degradation. If the newly synthesized protein is not rapidly phosphorylated or is dephosphorylated shortly after synthesis, the overall phosphorylation status may decrease despite an increase in the total protein level.
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