Navid J. Ayon

6 Questions 63 Answers 0 Followers

Questions related from Navid J. Ayon

Can the host of IP antibody and the WB primary antibody be same?

If I am doing an IP for a mouse and a human protein with an antibody that is raised in Rabbit and recognizes both the mouse and human protein, and later when I want to do WB, is it okay if the...

14 December 2021 5,452 3 View

What is an acceptable FDR% for proteomics data publication?

Is there an established convention on this? I have heard from experts that 1% FDR is recommended. I was wondering can we report protein IDs at 5% FDR. Is there any published guidelines on this? If...

26 August 2020 1,425 4 View

For the needle to go lower in a Shimadzu HPLC should I increase or decrease the needle stroke?

My sample volume is 50 uL and I am injecting 45 uL. I am using 250 uL insert. But it looks like the injection is not reproducible. The default needle stroke was 52 mm and I made it 51 mm...

05 October 2017 9,089 1 View

How to adjust the needle of HPLC so that it can reach the well of a 96 well plate kept in the autosampler?

LC-MS system comprised of QTrap3200 and a Shamidzu HPLC (Autosampler model is SIL-20A). The needle is not going into the well instead going in between two wells and not picking up the sample....

24 February 2017 1,504 4 View

Basic pH and HPLC

Does anyone have a good reference or can share their expert view on effect of pH on HPLC instruments? I know there are lot of resources available online on effect of pH of the mobile phase on...

01 January 1970 10,077 4 View

Any must-read literature for SEC method development?

I am new to SEC and developing this workflow for primarily separating Abeta oligomer from mice brain. However, there are other proteins of interest down the road. I was wondering if anyone can...

01 January 1970 2,814 3 View