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Questions related from Navid J. Ayon
If I am doing an IP for a mouse and a human protein with an antibody that is raised in Rabbit and recognizes both the mouse and human protein, and later when I want to do WB, is it okay if the...
14 December 2021 5,466 3 View
Is there an established convention on this? I have heard from experts that 1% FDR is recommended. I was wondering can we report protein IDs at 5% FDR. Is there any published guidelines on this? If...
26 August 2020 1,450 4 View
My sample volume is 50 uL and I am injecting 45 uL. I am using 250 uL insert. But it looks like the injection is not reproducible. The default needle stroke was 52 mm and I made it 51 mm...
05 October 2017 9,101 1 View
LC-MS system comprised of QTrap3200 and a Shamidzu HPLC (Autosampler model is SIL-20A). The needle is not going into the well instead going in between two wells and not picking up the sample....
24 February 2017 1,523 4 View
Does anyone have a good reference or can share their expert view on effect of pH on HPLC instruments? I know there are lot of resources available online on effect of pH of the mobile phase on...
01 January 1970 10,088 4 View
I am new to SEC and developing this workflow for primarily separating Abeta oligomer from mice brain. However, there are other proteins of interest down the road. I was wondering if anyone can...
01 January 1970 2,820 3 View