How can I prepare large unilamellar vesicles?

More Riccardo Leinardi's questions See All

How to solve issues with bender cuts in NLP?

hi. i'm trying to solve a NLP with bender cuts. at a certain point i have obtained one condition in the slave problem which is X>=0 and a second condition (an other cut) that states that...

12 November 2023 2,010 0 View

Latent Transition Analysis on R?

I need to perform a Latent Transition Analysis. My goal is to create different profiles using several cognitive variables and see if they change over time (e.g. a subject at time 1 is in Profile 1...

10 July 2023 7,667 2 View

Does an antibody made in mouse and conjugated with biotin react with a secondary antibody against mouse?

I have to perform an IHC and I have three different antibodies to test. We already have one made in rabbit and another made in mouse, both antibodies react against human. We have to test a third...

28 June 2023 4,750 4 View

How many samples should I analyze from a single batch of raw materials shipped in different drums/containers?

Hi there, I was wondering, from a QA/QC perspective, If I have one batch of raw material coming in the factory in big quantity, split in different drums or containers, how many samples should I...

11 June 2023 7,222 1 View

Calculate residual mass of an inorganic fiber from the ICP-MS result in murine lung homogenate?

I have analyzed 100 ug of mineralized asbestos fibers (volume: 50 ul) diluted up to 500 ul. After analysis on ICP-MS, I obtained X ppm of Mn. Then, I analyzed 50 ul of lung homogenate obtained by...

30 March 2023 6,073 1 View

Any model available for daily internet traffic load profile?

Hi, is there any model for daily internet traffic characterization? Or maybe can be available some update papers discussing the changes of internet traffic load during the day for xDSL, Fiber,...

29 August 2022 6,826 0 View

Unknown contamination (m/z 100 and 295-297) on Waters Acquity-QDa LC-MS, any idea what it might be?

Hello, We have been having contamination issues with our single quad LC-MS system (Waters Acquity Arc QSMR, Sample Manager FTNR, 2998 PDA, QDa). In particular, we observe an omnipresent species...

06 July 2022 7,003 4 View

Best free software or R package to draw complex (animal) pedigrees?

Dear all, I have to draw the complete pedigree of a high number (>40) of dogs in a study regarding genetic diseases, so I started drawing the pedigree using the R package kinship2. The...

29 June 2022 255 3 View

Is it possible scanning neurons coloured with golgi staining by the use of a confocal microscope equipped with spinning disk?

Can a microscope equipped with a spinning disk Scan a Sample by the use of a reflection modality?

31 March 2022 2,110 0 View

Does anyone know of a visual search dataset for small objects in natural images?

I'm looking for a dataset of natural images, with no specific requirement on the type of scene, designed for the task of detecting (automatically or visually) the presence and position of small...

26 January 2022 6,051 3 View

How to completely dissolve soy lecithin?

Hello, I'm looking to dissolve soy lecithin in order to create a liposome. I've tried using various solvents like pure ethanol, methanol, and a mixture of ethanol and methanol but they didn't...

02 August 2024 6,980 4 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

Comparing Liposome Size: DLS Measurement vs. SEM Observation with Air Drying ?

I have an issue with SEM imaging of liposomes. When measured by DLS, they appear to be around 120 nm, but when using SEM with simple air drying, they appear much larger, around one micrometer.

25 July 2024 3,395 3 View

How to dispose off lipids waste?

I am looking for a lipid waste disposal method, keeping in mind the environmental, health and safety aspect of lipid waste. Could someone please provide guidelines or the lowest acceptable...

25 July 2024 637 5 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 5 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 5 mg/mL?

18 July 2024 9,831 0 View

Why there is a absence of colonies on the Abx plate for selection after liposome mediated DNA transfer ?

Hi All I am looking to get your opinions, I have perfomed liposome mediated DNA transfer into bacteria, the DNA is a plasmid with msfGFP and when I induce the plasmid I see the fluorescence, and...

16 July 2024 4,698 4 View

Liposome Preparation using Heating Method - Why does my liposome suspension form aggregates?

Hello everyone, I am trying to encapsulate an essential oil in the liposomes using the heating method and encountering some issues. I am following the protocol described below, but my solution...

10 July 2024 4,083 3 View

Any better protocol for entrapment efficiency of curcumin?

I am currently trying to measure the entrapment efficiency of curcumin in liposomes. Typically, this measurement can be done by centrifugation, and I have followed the protocol outlined by Asif et...

01 July 2024 7,007 1 View

How to insert cholesterol inside DPPC membrane by replacing same number of DPPC molecules in GROMACS?

I am trying to insert cholesterol inside the DPPC membrane by using gmx insert_molecules -replace. But number of DPPC molecules replaced is much higher than the number of cholesterol inserted?...

30 June 2024 4,160 2 View

Jaime Guillén

Vesicles of 1-10 micrometers size are giant unilamellar vesicles (GUVs). You can form it by fil rehidration technique: http://www.avantilipids.com/index.php?option=com_content&view=article&id=1601&Itemid=382 or by electrofomation for example: http://www.nature.com/nprot/journal/v10/n1/box/nprot.2015.003_BX2.html

Francisco J Barrantes

Please find enclosed two relevant papers.

Best wishes. Francisco

Riccardo Leinardi

Thank you all for your precious comments.