Ideal samples packaging for edible oils?

Hello everyone, I have maybe a stupid question regarding the right packaging to use when comes to send some oil samples in external labs. Do you have any advice about that? Is there a risk that...

18 January 2021 7,826 4 View

Alternative to use of ethanol in extraction process?

Dear all., I am using ethanol as part of the extraction process of Chlorella vulgaris carbohydrates. Since I am trying to scale up a bit the whole process, I should use much more ethanol, that in...

27 August 2020 1,875 5 View

Do I have to activate NR8383 rat macrophages with LPS before incubation with particles and investigation of cytokines release?

Hello everybody, I'm planning to investigate the in-vitro release of different markers (CXCL10, INF-a, IFN-b, 1L-1a, IL-1b, IL-6, free DNA) by NR8383 rat macrophages, upon incubation with several...

20 August 2020 8,791 1 View

Do you know if Covid-19 dataset is available somewhere?

I'm searching for a numerical dataset about the virus. I found only daily statistical data but i would like access to single patients data. Does anyone know about it?

09 March 2020 6,626 85 View

How do I report stats for a zero inflated negative binomial model?

I need to report my statistical findings in a paper but I am struggling to report the output from R. how do I treat the two sections (Count model coefficients and Zero Inflation model...

28 January 2020 1,422 2 View

What statistical method would you use to analyse point count data on butterfly migration?

I have a set of data to analyse on butterfly migration. Unfortunately my stats skills are not as good as my field skills! Me and my peers have counted the amount of butterflies migrating in one...

09 January 2020 8,150 11 View

How to choose a suitable journal?

Dear researchers, I know some sites that can help to choose a suitable journal to a given manuscript, namely: journalfinder.elsevier.com journalsuggester.springer.com journalfinder.whiley.com can...

13 October 2019 1,453 3 View

Buffer without monovalent salts, can I use Tris?

18 April 2018 8,432 1 View

Alternative to PBS buffer with low salt concentrations?

Hello all, I find myself in need to perform serial dilution of E.coli cells for a luminometric ATP assay. Normally for serial dilution I would use PBS, but from what I understood the...

18 April 2018 5,359 3 View

Toxicity of H2O2 against coli?

Greetings, I am currently trying to determine the toxicity of the effluents of an Electro-Fenton reactor with macrotoxicity method and colony count. The concentrations of H2O2 I calculated...

13 January 2018 1,660 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View