Riccardo Leinardi

5 Questions 5 Answers 0 Followers

Questions related from Riccardo Leinardi

Calculate residual mass of an inorganic fiber from the ICP-MS result in murine lung homogenate?

I have analyzed 100 ug of mineralized asbestos fibers (volume: 50 ul) diluted up to 500 ul. After analysis on ICP-MS, I obtained X ppm of Mn. Then, I analyzed 50 ul of lung homogenate obtained by...

31 March 2023 6,090 1 View

Do I have to activate NR8383 rat macrophages with LPS before incubation with particles and investigation of cytokines release?

Hello everybody, I'm planning to investigate the in-vitro release of different markers (CXCL10, INF-a, IFN-b, 1L-1a, IL-1b, IL-6, free DNA) by NR8383 rat macrophages, upon incubation with several...

21 August 2020 9,036 1 View

Can anyone suggest me an operating protocol to detach THP-1 differentiated-macrophages for FACS-array analysis?

I'm trying to evaluate several cytotoxicity endpoints upon internalization of silica particles on macrophages differentiated from THP-1 cells, but we are having some issues with the detachment of...

27 March 2018 1,462 5 View

Does size affect liposome stability?

Considering two populations of liposomes, one set at ca. 220 nm, the other set at ca. 440 nm, made of the same phospholipid (e.g. phosphatidylcholine only, no cholesterol), which is, in physical...

21 September 2016 5,168 13 View

How can I obtain unilamellar liposomes of ca. 200 nm without using a lipid extruder?

I need to obtain a liposome dispersion of about 220-250 nm (using only egg yolk phosphatidylcholine) characterized by a low PdI (

22 February 2016 4,438 15 View