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Questions related from Riccardo Leinardi
I have analyzed 100 ug of mineralized asbestos fibers (volume: 50 ul) diluted up to 500 ul. After analysis on ICP-MS, I obtained X ppm of Mn. Then, I analyzed 50 ul of lung homogenate obtained by...
31 March 2023 6,097 1 View
Hello everybody, I'm planning to investigate the in-vitro release of different markers (CXCL10, INF-a, IFN-b, 1L-1a, IL-1b, IL-6, free DNA) by NR8383 rat macrophages, upon incubation with several...
21 August 2020 9,046 1 View
I'm trying to evaluate the impact of the internalization of unlabelled crystalline silica sub-micrometric crystals in THP-1 macrophages, through lysotracker red assay, by means of flow cytometry....
20 June 2018 3,064 2 View
I'm trying to evaluate several cytotoxicity endpoints upon internalization of silica particles on macrophages differentiated from THP-1 cells, but we are having some issues with the detachment of...
27 March 2018 1,466 5 View
Considering two populations of liposomes, one set at ca. 220 nm, the other set at ca. 440 nm, made of the same phospholipid (e.g. phosphatidylcholine only, no cholesterol), which is, in physical...
21 September 2016 5,175 13 View
I need to obtain a liposome dispersion of about 220-250 nm (using only egg yolk phosphatidylcholine) characterized by a low PdI (
22 February 2016 4,440 15 View
I would like to prepare unilamellar liposomes of, about, 1-10 micrometers, using, as lipid, only soy PC (Sigma) or a mixture of PC & cholesterol; can anyone recommend a functioning method?
28 September 2015 6,684 3 View
