Dear colleagues, I want to know how can I prepare the Giemsa stain as the last time I prepared it , it was light, not stable, and be faint after 2 days
Dear WA Gadalla,
when logged in to Research Gate, just copy and paste
https://www.researchgate.net/search.Search.html?type=question&query=Giemsa in the browser: Then you'll find all those threads, Q&A's already saved on RG-Archive...
(or you use directly the SEARCH function in RG: find rectangle field SEARCH upper menu line, click the downpointing arrow, choose "Questions from the menu and fill in the search word/phrase - then click the loupe icon and allow some second for results....) .just as a first orientation.
Since you unfortunately have NOT specified what purpose the GIEMSA staining solution should serve it might be essential and of benefit if you let readers know what you are going to stain with....(There are several "Giemsa-stains" and "Giemsa-Modifications", so the more accurate the details of your task, the more readers' answers will be on the spot...) Best regards, Wolfgang
Dear Gadalla,
I do not know exactly what you are going to stain, but the quality of the dye (GIEMSA) and the solvents are important. The quality depends on the manufacturer. It is also important stores the Giemsa solution in the amber botlle, avoidind direct expositure to light. Good luck, Ida
Just to motivate WA Gadalla to have a look into specific literature :
cf / go through only three threads in RG, which can be found @:
https://www.researchgate.net/post/can_anyone_give_the_protocol_of_giemsa_satining_of_tissue?
https://www.researchgate.net/post/Is_there_a_Giemsa_Stain_protocol_that_doesnt_require_at_least_two_months_to_let_stand? and
https://www.researchgate.net/post/What_is_the_procedure_for_Stock_Giemsa_Stain_preparation_and_Working_solution_to_analyze_human_metaphase_chromosomes
NB: Naturally quality of the dye (and other chemicals to be used), its/their source/manufacturer, storage mode and staining conditions are important parameters for successful staining. As for the quality of stain/dye one should read about the certification of dye(s) (e. g., by the ).
Thanks to Valentin Neubert, Philipps University of Marburg and
Carvalho L.O.P., Fundação Oswaldo Cruz for their recommendation of my reply.
Best of luck, WM.
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