Could you please help me to know if this digested DNA is good enough to detect SNP or it must be done again?

Hello everyone I hope all is well I want to know what is wrong with my experiment as DNA was extracted and after digestion by restriction enzyme it gives a very faint bands and also I want to...

05 June 2019 4,911 1 View

How can I prepare Giemsa stain?

08 September 2017 9,783 3 View

How can i use medcalc software to calculate sensitivity , specificity , ppv, npv of Combinations of 2 markers ?

04 May 2017 7,089 1 View

Is there any reason that make protein show difference between groups but no difference have been showed by real time PCR?

Hello everyone, when I studied certain protein in patients who had different stage of liver disease (HCV, liver cirrhosis, HCC) and control group, I surprised that ELISA showed difference in the...

02 March 2017 7,600 3 View

What is the reason that cause RNA dose not appear on the gel?

10 November 2016 4,267 6 View

What is the protocol of using DEPC in RNA extraction ?

I want to extract RNA by Direct-Zol RNA Miniprep kit from sigma but the kit didn't provide RNase free tubes and one of my colleagues advised me to use diluted DEPC to sterilize normal Eppendorf...

09 October 2016 9,304 7 View

I want to ask about the validity of my samples?

Good evening, everyone. I hope all is well I want to ask about the validity of my samples, since they were Reserved as a whole blood since 2012 on EDTA tube (CBC tube with purple cap) at -20C and...

08 September 2016 8,005 7 View

Why blank value is higher than sample value in ELISA test? and Is there any way to avoid negative values after substract sample value from blank?

blank 0.208 values of samples ranges from (0.165-2.2080)a lot of sample values below blank value

04 May 2016 7,486 16 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to upload pdf of my articles published?

How to upload pdf of my articles published?

03 March 2021 2,183 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Would adding books published in Lambert Publishers increase my RG impact?

There are some books published in LAP. Shall I add it to the RG?

02 March 2021 2,753 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is Journal of Agricultural Research a predator?

it is published by Juniper

28 February 2021 354 4 View

How can I publish a manuscript in free or low cost journal with good H-Index?

Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...

28 February 2021 7,800 6 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View