How mirna chose its target among 100s of mRNA having seed sequence?

I am not clear what is rule behind mirna and mRNA targeting pair,because each mirna target officially 100s of target. In such cases how one will validate legitimate mirna mRNA target. Assays...

21 April 2019 281 4 View

Films prepared by combination of Hypromellose E15 and E5 polymer with 20% of plasticizer (PEG 400) w.r.t dry polymer using water results in brittle ?

This question is related to soluble film formulation.

04 September 2018 3,086 1 View

How to meassure primary miRNA trancript using QRT PCR?

I want to know how to design primer for primary miRNA QRT PCR

05 March 2018 4,842 6 View

How to draw Scatchard plot, using data of fluorescence intensity and concentrations of ligands and protein only?

How to draw Scatchard plot, using data of fluorescence intensity and concentrations of ligands and protein only. Please help..

22 January 2018 992 4 View

I wanted to see if all the morphs of nematodes are same species or not by molecular sequencing, how to proceed doing it?

20 March 2017 5,885 3 View

What is the function of acetic acid and methanol in staining-destaining of sds page?

function of acetic acid and methanol in staining-destaining of sds page

17 February 2017 3,392 4 View

Can miRNA mimics or inhibitor used in primary cells?

I am currently working with lymphocyte ,I want to know if any one has used the miRNA mimics or inhibitor with primery cells

02 November 2016 1,753 3 View

How to reduce size of a void in a metal from micron to nanometer range ??

I want to know if there is a way in which the void of the material inside the metal can be divided from micron size to nanometer size by splitting the voids into discrete voids of reduced size...

03 October 2016 6,811 3 View

How is the molecular phylogenetic analysis of foraminifera beneficial for taxonomic studies?

I would like to know the actual role of rDNA and protein sequences in evolutionary relationships of foraminifera.

23 August 2016 9,210 2 View

Has anyone implemented Self Organizing (Adaptive) Fuzzy controller?

I have read a lot theory about Self Organizing Fuzzy control from Internet. But I am still not completely able to understand its theory and implementation.I have attached one of the best document...

02 June 2016 2,438 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View