How can I perform microRNA transfection in ovo?

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Is it necessary to covary exogenous constructs in a structural model?

I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...

03 August 2024 6,028 4 View

How to get the band.dat (band) files in phonopy calculations using VASP?

Hello Dear All I am a new user of VASP (Vienna ab-initio simulation packages) and tried to calculate the phonon using VASP. I get the phonon band structure (after calculations) in image form(.png...

11 December 2023 9,204 6 View

How to check immunogenicity and cytotoxicity of a drug delivery system like PLGA/PEG using in-vitro methods?

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12 July 2023 6,139 0 View

Call of Editorial boards at Algerian Journal of Biosciences?

Call of Edotrial boards in Algerian Journal of Biosciences About more informations please visit the journal website https://www.journal.acse.science/index.php/ajb/index If you are interested...

17 April 2023 3,999 0 View

Is it possible to detect PAHs directly from microplastic particles found in fish guts and gills?

I have already processed my fish gut samples for microplastics analysis from extraction to filtration and drying. The extracted microplastic particles were stored in dried glass fiber membranes....

16 April 2023 8,457 2 View

Stock solution of α-Amylase Porcine Pancreatic powder (Megazyme)?

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29 March 2023 3,397 0 View

Is there a software that can represent the hcp crystallographic orientation, and give a quantitative analysis of these orientations given an EBSD ?

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18 March 2023 3,753 0 View

How do electrons transport (electrical conductivity) in layered materials (e.g. Graphene or h-BN) in out-of plane direction?

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01 February 2023 8,315 2 View

How to conduct a systematic literature review?

How to conduct a systematic literature review (SLR)? What sould it include? Could you share resources including guidlines for writing an SLR? What is the difference between an SLR and a regular...

03 January 2023 10,024 4 View

Is there any way (metric) available that can help to quantify how difficult it will be to sing a song?

I want to build a song recommendation engine based on the difficulty of singing a song. I am unable to find any prior research or method of evaluation for a song. Help much appreciated.

28 November 2022 1,892 13 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

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06 August 2024 1,495 2 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

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Weak DAPI staining after immunohistochemistry - how to improve?

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Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

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04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

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03 August 2024 3,371 3 View

Interested in a SCOPUS collaboration?

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02 August 2024 7,843 0 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

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02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

M. Ricky Ramadhian

http://www.jove.com/video/50024/in-ovo-expression-of-microrna-in-ventral-chick-midbrain

maybe you can open this

Alia Abukiwan

Hallo Lale,

Thanks, I am using mimics from Life technologies and they are working good in vivo

I am asking for the amount of the mimics and the protocol (How much mimics with transfection reagent should I add pro Egg)