Alia Abukiwan

6 Questions 14 Answers 0 Followers

Questions related from Alia Abukiwan

How can I perform microRNA transfection in ovo?

Hallo I am going to transfect my miRNA candidates into Chicken egg treated with a drug. I'll use filter paper, but I still not sure of the miRNA amount in the transfection reagent Any help? Any...

08 December 2014 3,765 2 View

Does anybody familiar with inducing EMT by using TGFb recombinant Protein?

Hello, I need to induce EMT by TGFb recombinant Protein, and also I want to transfect the X cell line with my miRNA candidate and inhibitor. I need an efficient protocol please! First inducing...

12 November 2014 6,054 3 View

Can anyone recommend a protocol for invasion assay & migration?

Hello all, I want to express miRNA candidates in the PC cell line, and I would like to know what is the best protocol for cell numbers and the miRNA conc. Thanks for the information.

15 August 2014 9,324 3 View

Does anyone have experience with miRNA Taqman (stable control)?

Hello miRNA experts, I am doing a miRNA Taqman assay. The Ct values for all miRNA candidates are fine. But I have a problem with reference miRNA (Control ), I used three different controls: RU6B,...

01 August 2014 9,055 3 View

What is the consensus binding motif of Dexamethasone?

I want to know if Dexamethasone serves as either a transcription factor to enhance or repress transcriptional regulation. Which bioinformatic program can I use?

26 May 2014 6,036 6 View

How can I pick the best miRNAs that target my gene/singling pathway of interest?

The microRNA profiling had done for cancer cells treated with (X) drugs, and we want to understand the mechanisms of this drug. A lot of functional experiments were done previously in my lab, and...

26 May 2014 5,829 5 View