Leaf of Camellia sinensis is very much thick, waxy and rigid one. Peeling a good amount of the epidermal surface for the visualization of Real time NO generation using DAF-2DA dye or visualization of Callose deposition is very much essential for my work. Please suggest me if any alternative solution of the problem is available to you.
@ Phillip Morris: We just hold a leaf upside down and tear it by pulling it upward from an edge with a forceps.
@ Bergounioux Catherine: Plants are planted in the open environment, not in a greenhouse. So maintaining short days is not possible. Moreover, I do not understand how it would solve my problem.
@ Phillip Morris: Thank u very much for your kind answer. I will definitely give a try to your methods. Hope any one of them will work for me.
- Badges
- Science method