For my experiment I am using quercetin and kaempferol as standards. I am unable to dissolve them in water or methanol, or even in 50% methanol water. Please suggest any alternative solutions for this. I am using water and ACN as a mobile phase in HPLC.
Dear Damodar,
One issue we commonly encounter, while using DMSO to dissolve sample for HPLC, is 'peak dragging' and unsymmetrical peaks. It is better to avoid DMSO as much as possible
According to the Merck Index, kaempferol is soluble in hot ethanol, ethyl ether, acetone, acetic acid, and alkalies. Quercetin is soluble in ether, methanol, ethanol, acetone, pyridine, and glacial acetic acid. As for compatibility with your instrument or method, that is your call.
http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?cid=5280343#x27
http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?cid=5280863#x27
I am not sure what concentration of Kaempferol and Quercetin you need.
Refer USP36 - Powdered Ginkgo leaf extract monograph - this gives you the actual std solution concentration for HPLC. 0.125 mg of Kaempferol in 1 mL methanol; 0.125 mg of quercetin in 1 mL methanol. This is a time tested method of standard preparation (of Kaemperferol) for HPLC and we had been routinely preparing kaempferol solutions without any hitch.
All the best
You can try as suggested by Suresh Govindaraghavan. Alternatively, try dissolving in DMSO and dilute with methanol to get your final concentration.
Dear Damodar,
One issue we commonly encounter, while using DMSO to dissolve sample for HPLC, is 'peak dragging' and unsymmetrical peaks. It is better to avoid DMSO as much as possible
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