No title

02 January 2021 7,417 10 View

What could be the cause of the Glutathione peak migration (LC Dionex, Kintetex EVO C18, water phase) ?

Hello, I need a help from those of you who work on Kinetex EVO C18 with water + 0.2% TFA mobile phase (isocratic). The conditions are constant (temperatures, flow, column, conditioning time)....

16 December 2020 4,582 3 View

How does one combine the basis of Quantum Physics that the information cannot be destroyed with the GR statement that black holes destroy the info?

How does one combine the basis of Quantum Physics that the information cannot be destroyed with the GR statement that black holes destroy the info?

14 December 2020 7,845 19 View

Macrostudies using sysGMM?

Dear all, Could you recommend macroeconomic studies (hopefully published in good journals) that use system GMM or Arellano-Bond estimators? Thanks a lot!

10 December 2020 5,130 4 View

What do you think about the article in Scientific American that nobody knows how planes lift off and fly?

https://www.scientificamerican.com/article/no-one-can-explain-why-planes-stay-in-the-air/

07 December 2020 4,404 19 View

No title

07 December 2020 9,722 12 View

Why my Nyquist plot does not intersect the x-axis?

I have been measuring the conductivity of the biopolymer membrane by using the 2-electrodes connection and sometimes the plot intersects the a-axis and other times doesn't. The membrane was...

03 December 2020 8,471 1 View

GPower for Three-Way ANOVA analysis?

I want to explore if the amount of time (usage) spent on the Social Media IV1, with two levels , one is light and the other is heavy use, Iv2, body image with negative and positive levels, Iv3...

17 November 2020 4,405 3 View

DNA extraction from bacterial culture?

Is it an effective method to isolate DNA from Gram-negative bacteria with only the use of a boil and freeze-thaw protocol, without any chemical purification? Also, is there a media to maintain the...

06 October 2020 6,890 8 View

Is there an equation connecting wave function and entropy of the quantum system?

Is there an equation connecting the wave function and the entropy of the quantum system?

27 July 2020 931 13 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Will it be possible to get IC:50 if we target cancer cell line with antibody drug conjugate (ADC) that have 50% less antigen positive cells ?

Is it possible to attain IC50 if my target cells have less than 50% antigen expression ? For example: Cancer cell line XXX is showing 35% of expression of cell surface antigen YYYY, if i...

04 February 2021 2,711 3 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

How to use multiple tables to generate a graph in Graphpad Prism?

I have two different tables: one for metabolic activity of the cells in a plate, the other one is the Total DNA assay from the same plate. I need to generate a graph which represents metabolic...

29 January 2021 3,294 5 View

IC50 value from this graph?

01 January 2021 6,848 4 View

I am performing enzyme inhibition assay and the substrate is fluorogenic & fluorescence was measured. Now I want to calculate Vo and Vi. How can I ??

These are the per minute fluorescence observed. Please guide me how can I determine Vi, Vo and IC50 ? By slope determination or any other method? Control: 28.11 40.30 40.78 37.61...

11 December 2020 1,037 4 View

How to create a metal binding curve for a metalloprotein using prism?

hello, I need to create a binding curve to measure the affinity of my enzyme to its zinc cofactor, to determine the Kd value. I'm using prism (graphpad) and there are a lot of options under the...

14 November 2020 2,120 3 View

Need help plotting long lists of data in Graphpad Prism -?

Hi, I am generating long lists of per-cell data that I'd like to visualize as dot plots per condition. The problem is that my conditions are defined by letters rather than numbers. Or sometimes I...

11 November 2020 6,707 1 View

How to determine non-cytotoxic drug concentration using MTT assay?

Many literature showed that how to determine IC50 drug concentration using MTT assay. But I want to know how to determine non-cytotoxic concentration of a particular drug with maximum its...

31 October 2020 2,012 3 View

Can I display regression line for a whole group, when subgroups are displayed on Graphpad Prism?

I have created a scatter graph in Prism under -> multivariable analyses -> extract and re-arrange, to display relationship between 2 variables, the 3 subgroups within the group are...

16 October 2020 310 1 View