I am testing different combinations of peptides grafted to alginate hydrogels. Inside these hydrogels I have culture human mesenchymal stem cells and now I want to see how the different combinations of peptide influence the cell behavior. For that, I would like to understand whether cell volume changes as well as cell major length, sphericity or other measurement that can describe cell geometry. I've been trying to use Amira-avizo to measure diameter/length (approximated from a 2D disc) of cells that have been 3D reconstructed. I already managed to get all these values but they do not correlate very well with the 3D reconstructions that I've obtained from my different conditions. Is there anyone ho know how to use this Amira for this purpose for this purpose or have suggestions for other software ?