Hello! I want to measure the osmotic pressure/osmolarity of some tissue culture media. I have a freezing point osmometer which I use for liquid media, however I don't know if this will be appropriate for tissue culture media as it contains agarose (0.8% w/v). I have considered measured the media before addition of agarose, but I am not sure if the agarose would change the outcome. Does anyone know how to handle this?
Thanks :D