Hi all,
For my plasmid isolation the protocol says to use 2 M acetic acid but our store has only got 100 % pure glacial acetic acid. So how can I make a 2 M acetic acid? Happy for any help!
Glacial acetic acid is 17.4M. From that, you can use the M1V1=M2V2 (where M=molarity and V=volume) equation to calculate the volume of glacial acetic acid you'd need for however much 2M you're making. So:
17.4M x (Volume of glacial acetic acid needed) = 2M x (Volume of 2M acid you need)
Hi Erin,
17.4 M of glacial acetic acid is the value I was looking for. Thanks for helping me!
For more clarity visit following;
http://www.sigmaaldrich.com/chemistry/stockroom-reagents/learning-center/technical-library/molarity-calculator.html
Hell Erin Brettmann, How I know that the provided glacial acetic acid is 17.4M.
Hi all,
thanks to Erin, Anastasia and others. Your comments were really helpful and it worked out fine.
All the best to you!
Use general formula that is
(Molecular weight x Required Concentration x Required Volume x100)/(1000 x Density x Assay).
For example, to prepare 1L of 2 Mole of Acetic acid:
(60.05 X 2 X 1000mL X100)/( 1000 X 1.049 X 99.7) = 114.834 ml
that means 114.834 ml of glacial acetic acid +885.165 mL of water (makeup to 1L)
Natasa Mantziari n=m/Mr : what does m and Mr refers to? how come its 1049 g / 60.05 g/mol?
F=10×p(purity)×d(density)/fw(formula weight)
p=100%
d=1.05 g/cm³
fw=60.052 g/mol
==>
F=10×100×1.05/60.052=> F=17.48M
We suppose that you are going to make 25 ml acetic acid 2M.
m1v1=m2v2
2×25=17.48v2=> v2=2.86 ml
Dilute 2.86 ml of 100% pure glacial acid to the total amount of 25 ml in a volumetric flask with deionized water. (or simply add 22.14 ml of deionized water to 2.86 ml of acetic acid)
@David Thanam: what do you mean by assay and how did you get 99.7
http://www.sigmaaldrich.com/chemistry/stockroom-reagents/learning-center/technical-library/molarity-calculator.html
@Yemisi Omonije: Assay is nothing but purity in percentage and usually it is labeled in chemicals nameplate or you can get it in the manufactures product webpage.
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